Crystallization and preliminary x-ray diffraction study of cholera toxin B-subunit.

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Crystallization and preliminary x-ray diffraction study of cholera toxin B-subunit.

Cholera toxin binds to its ganglioside GM1 receptor via its B-subunit, a pentameric assembly of identical subunits (Mr = 11,600). Diffraction quality crystals of cholera toxin B-subunit have been obtained at room temperature by vapor diffusion with polyethylene glycol in the presence of the nonionic detergent beta-octyl glucoside. The crystals have been characterized with x-radiation as monocli...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1988

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(19)76569-7